Detection of four imperiled western North American freshwater mussel species from environmental DNA with multiplex qPCR assays

Publication Type:

Journal Article

Source:

bioRxiv (preprint for Freshwater Science article), Issue 012088v1, p.1-21 (2020)

Call Number:

A20ROD01IDUS

URL:

https://doi.org/10.1101/2020.03.27.012088

Keywords:

Anodonta nuttalliana, Anodonta oregonensis, freshwater mussles, Gonidea angulata, Margaritifera falcata

Abstract:

We developed multiplexed, species-specific, quantitative PCR assays for the detection of four freshwater mussel species native to western North America, Gonidea angulata, Margaritifera falcata, Anodonta nuttalliana and Anodonta oregonensis, from environmental DNA (eDNA). These species have experienced dramatic declines over the last century and are currently threatened in many portions of their ranges. Therefore, improved tools for detecting and monitoring these species are needed. Species-specificity and sensitivity of assays were empirically tested in the lab, and multiplex assays were also validated with field collected eDNA samples. All assays were species-specific, sensitive, and effective for detection from eDNA samples collected from streams and rivers. These assays will aid in the detection, monitoring, management, and conservation of these vulnerable species.

Notes:

Electronic file - Zoology: Invertebrates
"This document is a US Government work. It is not subject to copyright under 17 USC 105 and is also made available for use under a CC0 license."
NOTE also that this article is a preprint version as I cannot currently get to the Journal. The currently saved preprint is not peer reviewed. If print-published document is acquired, the document could be filed and this Biblio record revised.