Using herbarium-derived DNA s to assemble a large-scale DNA barcode library for the vascular plants of Canada

Publication Type:

Journal Article

Authors:

Maria L. Kuzmina; Thomas W. A. Braukmann; Aron J. Fazekas; Sean W. Graham; Stephanie L. Dewaard; Anuar Rodrigues; Bruce A. Bennett; Timothy A. Dickinson; Jeffery M. Saarela; Paul M. Catling; Steven G. Newmaster; Diana M. Percy; Erin Fenneman; Aurélien Lauron-Moreau; Bruce Ford; Lynn Gillespie; Ragupathy Subramanyam; Jeannette Whitton; Linda Jennings; Deborah Metsger; Connor P. Warne; Allison Brown; Elizabeth Sears; Jeremy R. Dewaard; Evgeny V. Zakharov; Paul D. N. Hebert

Source:

Applications in Plant Sciences, Volume 5, Issue 12 (2017)

Call Number:

A17KUZ01IDUS

Keywords:

Canadian vascular flora; DNA barcode reference library; herbarium; ITS2; matK; rbcL

Abstract:

<em>Premise of the study</em>: Constructing complete, accurate plant DNA barcode reference libraries can be logistically challenging for large-scale floras. Here we demonstrate the promise and challenges of using herbarium collections for building a DNA barcode reference library for the vascular plant flora of Canada.

<em>Methods</em>: Our study examined 20,816 specimens representing 5076 of 5190 vascular plant species in Canada (98%). For 98% of the specimens, at least one of the DNA barcode regions was recovered from the plastid loci rbcL and matK and from the nuclear ITS2 region. We used beta regression to quantify the effects of age, type of preservation, and taxonomic affiliation (family) on DNA sequence recovery.

<em>Results</em>: Specimen age and method of preservation had significant effects on sequence recovery for all markers, but influenced some families more (e.g., Boraginaceae) than others (e.g., Asteraceae).

<em>Discussion</em>: Our DNA barcode library represents an unparalleled resource for metagenomic and ecological genetic research working on temperate and arctic biomes. An observed decline in sequence recovery with specimen age may be associated with poor primer matches, intragenomic variation (for ITS2), or inhibitory secondary compounds in some taxa.

Notes:

Reference Code: A17KUZ01IDUS <br>

Full Citation: Kuzmina, M. L., T. W. A. Braukmann, A. J. Fazekas, S. W. Graham, S. L. Dewaard, A. Rodrigues, B. A. Bennett, T. A. Dickinson, J. M. Saarela, P. M. Catling, S. G. Newmaster, D. M. Percy, E. Fenneman, A. Lauron-Moreau, B. Ford, L. Gillespie, R. Subramanyam, J. Whitton, L. Jennings, D. Metsger, C. P. Warne, A. Brown, E. Sears, J. R. Dewaard, E. V. Zakharov, and P. D. N. Hebert. 2017. Using herbarium-derived DNA s to assemble a large-scale DNA barcode library for the vascular plants of Canada. Applications in Plant Sciences. Published by the Botanical Society of America. 11 p. <br>

Location: ELECTRONIC FILE - BOTANY: OTHER <br>

Keywords: Canadian vascular flora; DNA barcode reference library; herbarium; ITS2; matK; rbcL <br>